A killer factor produced by the cellular slime mold Polysphondylium pallidum |
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Authors: | Akiko Mizutani Hiromitsu Hagiwara Kaichiro Yanagisawa |
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Affiliation: | (1) Institute of Biological Sciences, University of Tsukuba, 305 Ibaraki, Japan;(2) Department of Botany, National Science Museum, 305 Tsukuba, Ibaraki, Japan |
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Abstract: | A killer strain was discovered in cellular slime molds. The wild isolate CK-8 of Polysphondylium pallidum kills all other strains in Polysphondylium and Dictyostelium, as far as could be determined, except strain CK-8 itself and its complementary mating type strain CK-9. Growth-phase cells of CK-8 excrete a killer factor which is sensitive to heat, above 60°C for 5 min, and trypsin. The apparent molecular mass of the factor was determined at 10 000–12 000.Abbreviations BSS Bonner's salt solution - CM conditioned medium |
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Keywords: | Killer strain Killer factor Cellular slime mold Polysphondylium pallidum |
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