Mating reaction in Saccharomyces cerevisiae VI. Effect of 2-deoxyglucose on conjugation |
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Authors: | Shimoda Chikashi; Yanagishima Naohiko |
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Institution: | Department of Biology, Faculty of Science, Osaka City University Sumiyoshi-ku, Osaka 558, Japan |
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Abstract: | The effect of 2-deoxyglucose (2-dG) on the mating reaction ofSaccharomyces cerevisiae was investigated and the followingresults were obtained. 1) The cell fusion process of the mating reaction was completelyinhibited by 0.05% 2-dG added to a culture medium containing2% D-glucose. This inhibition was partially reversed by raisingthe glucose concentration in the medium. 2) Sexual cell agglutination was hardly affected by 2-dG. 3) 2-dG at concentrations inhibiting cell fusion considerablysuppressed the incorporation of 14C-glucose into the cell wallpolysaccharides, glucan and mannan. 4) Glucose uptake and protein synthesis were only slightly inhibitedby 2-dG. 5) No enhancement of bulk polysaccharide synthesis was detectedduring mating.
1Present address: Biological Institute, Faculty of Science,Nagoya University, Chikusa-ku, Nagoya 464, Japan. (Received April 20, 1974; ) |
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