Transport of proteins from cytoplasm into plastids in chloramphenicol-treated bean leaf discs |
| |
Authors: | Kazimierz Strza?ka Maria Kwiatkowska |
| |
Institution: | (1) Department of Plant Biochemistry, Institute of Molecular Biology, Jagellonian University, Grodzka 53, 31-001 Krakôw, Poland;(2) Department of Plant Cytology and Cytochemistry, Institute of Physiology and Cytology, University of ód, S. Banacha 12/16, 90-237 odz, Poland |
| |
Abstract: | Leaf discs from etiolated bean plants were found to incorporate 3H]lysine into 80 S ribosomesynthesized proteins in the presence of chloramphenicol (100 mg l–1) when exposed to light. After a 7 min pulse of 3H]lysine, the discs were transferred to the same medium but with nonradioactive lysine, and postincubation was carried out for 24 h. The number of silver grains over the plastids, after the first period of a lag phase, indicates a large increase between 12 and 24 h of postincubation. Simultaneously, the labeling of the cytoplasm becomes reduced during that period. The results show that during inhibition of the protein formation within plastids, the synthesis of plastid-destined proteins in cytoplasm, as well as their transport into plastids, can still proceed. |
| |
Keywords: | Autoradiography Chloramphenicol Phaseolus Plastids Protein synthesis Transport |
本文献已被 SpringerLink 等数据库收录! |
|