Prolonged production of hydrogen gas by a chloroplast biocatalytic system. |
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Authors: | K K Rao L Rosa D O Hall |
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Affiliation: | University of London King''s College, Department of Plant Sciences, 68 Half Moon Lane, London SE24 9JF, U.K. |
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Abstract: | The evolution of H2 gas in an illuminated chloroplast plus hydrogenase system was shown to function for six and a half hours at a continuous rate of about 10 μmoles H2/mg. chlorophyll/hour. Chloroplasts from various plant species, using different isolation media, and storage in a fixed state (glutaraldehyde) at 4°, ?5° and ?196° were shown to catalyze H2 production. Both and hydrogenase were used. From the use of the photosystem II inhibitors DCMU and DBMIB and heat inactivation of photosystem II, it was concluded that H2O was the source of electrons for H2 gas production. |
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Keywords: | To whom enquiries should be addressed. |
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