无核荔枝果实形成差异表达基因cDNA的克隆

采用抑制差减杂交技术(Suppression Subtractive Hybridization,SSH)分离与海南无核荔枝果实形成相关的差异表达基因的cDNA片段,为克隆相关基因提供研究基础。分别以无核荔枝的有核幼果为driver;无核幼果为tester,建立差减cDNA文库。经Reverse Northern Dot-Blot筛选该文库,共获得61个阳性克隆,随机选取17个克隆进行测序,共获得10条非重复序列,对其中较长的7个序列进行同源分析,结果表明:有6个序列在荔枝中为首次报道。

Cloning the cDNA fragments of differentially expressed genes between seeded and seedless fruit of seedless litchi

The Suppression Subtractive Hybridization(SSH)technique was used to clone the cDNA fragments of differentially expressed genes between seeded and seedless fruit in Hainan seedless Litchi. One positive differential expressing cDNA library was constructed using the cDNA of seeded fruit as driver and the cDNA of seedless fruit as tester. Sixty-one positive clones were obtained by using the Reverse Northern Dot-Blot to screen the cDNA library. Nucleotide sequences of seventeen randomly chosen positive clones were sequenced and found ten are different sequences. Subsequently sequence analysis shows that the 6 clones corresponding to their nucleotide sequences all are first reported in Litchi varieties.