BmK AS在大鼠脑和蟑螂神经索突触体上的药理结合特性

用ＩＮＤＯＧＥＮ法对一个新型东亚钳蝎活性多肽ＢｍＫ ＡＳ进行了＾１２５Ｉ标记。分别在哺乳动物和昆虫标本上观察了标记物的药理结合特性，结果表明ＢｍＫ ＡＳ大大鼠脑突触体标本上有单一非协同结合位点，其平衡解离常数Ｋｄ与最大结合容量Ｂｍａｘ分别为１．４９ｎｍｏｌ／Ｌ，１．３９ｎｍｏｌ／ｇ。此外，ＢｍＫ ＡＳ在蟑螂神经索突触体标本同样也只有单一非协同结合位点，其平均解离常数Ｋｄ与最大结合容量Ｂｍａｘ分别为

Characteristics of BmK AS Binding to Rat Brain and Cockroach Nerve Cord Synaptosomes

BmK AS, one novel neurotoxic polypeptide from Chinese scorpion Buthus martensi Karsch was labeled by 125 I with INDOGEN method. Iododerivative was used in binding experiments on mammal and insect synaptosomes. BmK AS binds specifically to a single class of non interacting binding sites with high affinity ( K d=1.49 nmol/L) and low capacity ( B max =1.39 nmol/g) on rat brain synaptosomes. Likewise, BmK AS also binds specifically to a single class of non interacting binding sites with high affinity ( K d=0.79 nmol/L) and low capacity ( B max =6.60 nmol/g) on cockroach nerve cord synaptosomes. It indicates that there are special target receptors for BmK AS type scorpion neurotoxins on both mammal and insect excitable cell membranes.