| 应用自由流电泳分离小鼠脾淋巴细胞 |
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| 引用本文： | 刘韬,王升年.应用自由流电泳分离小鼠脾淋巴细胞[J].生物化学与生物物理学报,1999,31(5):590-593. |
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| 作者姓名： | 刘韬 王升年 |
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| 作者单位： | [1]中国科学院上海生物化学研究所 [2]中国科学院上海细胞生物学研究所 |
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| 摘 要： | 自由流电泳具有独特的全液相自由分离过程和非常温和的分离条件，使用这种技术进行细胞等水下溶性活性物质的分离不仅效率高，而且可以较好地保存其生物活性和成活力，自由电泳在低离子强度的三乙醇胺缓冲液系统中进行了Ｂ和Ｔ淋巴细胞的分离，并利用反向介质技术使分离后的细胞处于生理盐溶液中。
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| 关 键 词： | 自由流电泳 分离 淋巴细胞 成活力 |
Application of Free flow Electrophoresis to the Separation of Murine Lymphocytes |
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| LIU Tao,SHEN Xin,WU Gao De and XIA Qi Chang.Application of Free flow Electrophoresis to the Separation of Murine Lymphocytes[J].Acta Biochimica et Biophysica Sinica,1999,31(5):590-593. |
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| Authors: | LIU Tao SHEN Xin WU Gao De and XIA Qi Chang |
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| Institution: | LIU Tao,SHEN Xin,WU Gao De and XIA Qi Chang * |
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| Abstract: | Free flow electrophoresis offers not only the all in solution separation process but also very gentle separation conditions, and it can be used for continuous separation and preparation, so it has been widely applied in the fields of biochemistry and cell biology. Using this technique to separate insoluble particles such as cells, both high separation efficiency and high activity and viability could be obtained. Murine lymphocytes were separated in low ionic strength triethanolamine buffer by free flow electrophoresis, the cell fractions were detected and characterized by means of UV spectrometry, immune fluorescence labeling and flow cytometry. Results indicated that T and B lymphocytes were well separated with high viability. |
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| Keywords: | free flow electrophoresis triethanolamine buffer separation lymphocyte viability |
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