非洲爪蟾肌肉决定基因Xmyf—5表达调控的研究

Ｘｍｙｆ－５是爪蟾胚胎肌细胞决定的关键基因之一，研究Ｘｍｙｆ－５的表达调控有助于揭示肌肉原基的形成和肌肉发育的分子机制。从爪蟾部分基因组文库筛选到Ｘｍｙｆ－５５‘上游４．９ｋｂ片段。该片段指导报告基因在爪蟾胚胎内的表达以及其缺失片段指导的报告基因活性分析结果显示，Ｘｍ６ｓｆ－５５’上游４．９ｋｂ片段内含有指导Ｘｎｙｆ－５在胚胎内的表达以及其缺失片段指导的报告基因活性分析结果显示，Ｘｍｙｆ－５５‘

The Regulation of Xmyf 5 Gene, a Muscle Determinant, Expression in Xenopus Laevis

Xmyf 5 gene is a member of the myogenic regulatory genes important for Xenopus muscle cell commitment and differentiation. To analyze the mechanism involved in regulating Xmyf 5 gene activity in the process of myogenesis, the 4.9 kb 5' upstream sequence of Xmyf 5 was identified. Lac Z gene could be activated in the presomitic mesoderm in stage 14 embryos and myotomes in stage 25 embryos when it was driven by the 4.9 kb sequence and introduced into embryos at 2 cell stage. The Luciferase reporter gene activities driven by deletion constructs suggested that there are at least one enhancer between 4.9 kb and 3.5 kb of Xmyf 5 5' upstream DNA and one negatively regulative factor between 2.0 kb and 0.9 kb of the Xmyf 5 5' upstream DNA. In addition, sequence analysis indicated that a 100 bp fragment 5' upstream of Xmyf 5 contained conserved regulative elements such as OLS which were found in other vertebrates.