Study of the release of cell wall degrading enzymes during adhesion of Chlamydomonas gametes |
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Authors: | W J Snell |
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Institution: | Department of Cell Biology, The University of Texas Health Science Center at Dallas, Dallas, TX 75235, USA |
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Abstract: | A quantitative assay for cell wall release in Chlamydomonas has been used to study the timing of release of cell wall degrading enzyme (lysin) during adhesion. Lysin activity, which shows a broad pH range and requires divalent cations, is released as a pulse within 1–2 min after mixing of mt? and mt+ gametes. Thereafter, there is no further lysin release. Gametes of both mating types release the activity during aggregation with isolated gametic flagella of the opposite mating type, although mt+ gametes appear to release more lysin activity than mt? gametes. Electrophoretic analysis of cell wall proteins before and after lysin degradation indicate that the major wall proteins are unchanged after wall breakdown. |
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