Polyribosomes in protoplasts isolated from tobacco leaves |
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Authors: | P Ruzicska R Mettrie Y L Dorokhov G Premecz T Oláh G L Farkas |
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Institution: | (1) Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary;(2) Present address: Department of Cell Biology, University of Antwerp, Universiteitsplain 1, B-2610 Wilrijk, Belgium;(3) Present address: A.N. Belozersky Laboratory of Bioorganic Chemistry and Molecular Biology, Moscow State University, Moscow, USSR |
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Abstract: | Polyribosomes (polysomes), active in an amino acid incorporation system in vitro, were isolated from tobacco leaf protoplasts. A comparison of polysome profiles indicated that the polysome/monosome ratio is greatly decreased in isolated protoplasts as compared to the intact leaf. In isolated protoplasts, a marked accumulation of ribosomal subunits was also found. The division of protoplasts, as investigated in the 8-cell and callus stages, was associated with a(n) (at least) partial regeneration of polysome profiles characteristic for leaves. Plasmolysis of leaves attached to the plant had no great effect on the polysome profile. However, leaf excision per se resulted in a dramatic loss of polysomes, even when the leaf tissue was floated on water. It is concluded that the isolation of the cell from its normal environment, and not the osmotic stress and associated increase in RNase activity, is the most important factor responsible for the loss of polysomes in isolated protoplasts.Abbreviations EGTA
ethylene glycol bis (2-aminoethyl ether)-tetraacetic acid
- HEPES
N-2-hydroxyethylpiperazine-N -2-ethanesulfonic acid
- mRNA
messenger ribonucleic acid
- RNase
ribonuclease
- Tris
tris(hydroxymethyl)aminomethane
- TCA
trichloroacetic acid |
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Keywords: | Nicotiana Polysomes Protein synthesis Protoplasts Ribonuclease Ribosomes Stress |
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