首页 | 本学科首页   官方微博 | 高级检索  
   检索      

siRNA干扰MMP-9和FAK双基因抑制小鼠黑色素瘤生长和在体迁移
引用本文:胡娜,刘清,唐照勇,汤禾静,敖澜,赵紫豪,方廖琼.siRNA干扰MMP-9和FAK双基因抑制小鼠黑色素瘤生长和在体迁移[J].中国生物工程杂志,2016,36(5):34-39.
作者姓名:胡娜  刘清  唐照勇  汤禾静  敖澜  赵紫豪  方廖琼
作者单位:1. 西南大学生物技术学院 重庆 400716; 2. 重庆医科大学生物医学工程学院 省部共建超声医学工程国家重点实验室 超声医学工程重庆市市级重点实验室 重庆 400016
基金项目:国家"973"计划资助项目(2012CB722402)
摘    要:目的:观察干扰MMP-9和FAK双基因对恶性黑色素瘤高转移细胞B16F10体内转移的影响。方法:构建PGV102-MMP9-siRNA、PGV102-FAK-siRNA重组质粒载体,脂质体TM2000介导转染小鼠黑色素瘤B16F10细胞,RT-PCR检测基因的干扰效果;建立C57BL/6小鼠皮下移植瘤模型观察细胞在体成瘤和肿瘤的生长情况,常规组织切片,H&E染色观察肿瘤组织病理学特征;经C57BL/6小鼠尾静脉注射细胞5×105个/只,24天后计数小鼠肺转移结节数评价肿瘤细胞在体迁移能力。结果:RT-PCR结果表明,重组质粒转染细胞组的MMP-9和FAK的mRNA水平显著低于正常细胞组(P<0.01),转染细胞组C57BL/6小鼠皮下成瘤的肿瘤生长速率、黑色素瘤肺转移结节数明显低于正常细胞组(P<0.01)。结论:干扰B16F10细胞MMP-9和FAK双基因可明显抑制小鼠体内恶性肿瘤的生长和迁移。

关 键 词:转移  MMP-9  FAK  siRNA干扰  
收稿时间:2015-11-30

siRNA Inhibits the Growth and Migration of Mouse Melanoma by MMP-9 and FAK Gene
HU Na,LIU Qing,TANG Zhao-yong,TANG He-jing,AO Lan,ZHAO Zi-hao,FANG Liao-qiong.siRNA Inhibits the Growth and Migration of Mouse Melanoma by MMP-9 and FAK Gene[J].China Biotechnology,2016,36(5):34-39.
Authors:HU Na  LIU Qing  TANG Zhao-yong  TANG He-jing  AO Lan  ZHAO Zi-hao  FANG Liao-qiong
Abstract:Objective:To observe the effect of combined-silencing MMP-9 and FAK on migration of malignant melanoma highly metastatic B16F10 cells in vivo. Methods:Construct recombinant plasmid vectors pGV102-MMP9-siRNA and pGV102-FAK-siRNA, then Lipofectamine TM2000 mediated the plasmid vectors were transfected into B16F10 cells. To observe the growth of the tumor, the C57BL/6 mouse model of subcutaneous transplantation was established, and the pathological characteristics of tumor tissue was observed by H&E staining. B16F10 cells were stably intravenously injected into C57BL/6 mice by 5×105. After 24 days of injection, the number of mouse lung metastasis nodules was counted to evaluate the migration ability of tumor cells in vivo. Results:RT-PCR results showed that the mRNA level of MMP-9 and FAK in the transfected cells was significantly reduced than that of normal cells(P<0.01). Compared with the normal cells, the tumor growth rate and the number of lung metastasis nodules of C57BL/6 mice in the transfected cells were significantly reduced(P<0.01). Conclusion:Interference MMP-9 and FAK can significantly inhibit malignant tumor growth and metastasis in vivo.
Keywords:siRNA interference  MMP-9 FAK  Migration  
本文献已被 CNKI 等数据库收录!
点击此处可从《中国生物工程杂志》浏览原始摘要信息
点击此处可从《中国生物工程杂志》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号