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植物多基因转化研究进展
引用本文:初易洋,田慧琴,许蕙金兰,朱本忠. 植物多基因转化研究进展[J]. 中国生物工程杂志, 2016, 36(12): 111-116. DOI: DOI:10.13523/j.cb.20161216
作者姓名:初易洋  田慧琴  许蕙金兰  朱本忠
作者单位:中国农业大学食品科学与营养工程学院 北京 100083
基金项目:国家自然科学基金资助项目(31271959)
摘    要:通过转基因技术改良植物品质近几年已成为热点研究问题,基因工程不断发展,单基因转化技术已不能满足人们对植物改良的需要。更多的研究者投身于参与某个代谢途径的多个基因在植物体中共同表达的研究,通过多基因调控来获得更好的植物性状。基因的协调表达有四种研究思路,在此基础上多基因转化方法可概述为传统转化法、改进后的共转化法,及新兴的基因融合方法,综合分析每种方法在植物代谢调控中的优缺点与应用,并探讨多基因整合的不稳定及相互作用问题。

关 键 词:基因融合  植物  多基因转化  传统转化  
收稿时间:2016-06-14

Progress in Researches of Plant Multigene Transformation
CHU Yi-yang,TIAN Hui-qin,XU HUI Jin-lan,ZHU Ben-zhong. Progress in Researches of Plant Multigene Transformation[J]. China Biotechnology, 2016, 36(12): 111-116. DOI: DOI:10.13523/j.cb.20161216
Authors:CHU Yi-yang  TIAN Hui-qin  XU HUI Jin-lan  ZHU Ben-zhong
Abstract:Improving plant quantity traits through transgenic technology has been increasingly concerned in modern genetic transformation field.With the rapid development of genetic engineering,single gene transformation technology is insufficient for the plant modifications.In order to get better plant traits by multigene regulation,more researchers have focused on the study of genetic transformation,making the genes involved in metabolic pathways coexpression in plants.There are four genome expression routes for genes selection and basically plant genetic transformation methods cover the traditional and improved transformation methods,emerging polygene fusion.The advantages and disadvantages of each method in plant metabolism regulation and application progress were analyzed comprehensively and the future development was discussed.
Keywords:Multiple gene transformation  Polygene fusion  Traditional transformation  Plants  
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