异源表达CkLEA1基因增强了拟南芥对非生物胁迫的耐受性

植物在生长过程中会受到各种非生物胁迫的伤害，导致生长发育和产量受到严重影响，胚胎晚期丰富蛋白（late embryogenesis abundant proteins，LEA蛋白）在植物抵抗非生物胁迫过程中起着重要的保护作用。在前期的研究基础上，将受多种胁迫诱导的柠条锦鸡儿CkLEA1（GenBank登录号KC309408）基因转入野生型拟南芥，通过实时荧光定量PCR从7株T₃代纯合体中筛选出3个转基因株系做进一步研究。种子萌发率实验发现，在200 mmol/L NaCl和400 mmol/L甘露醇处理下，转基因株系萌发率均高于野生型拟南芥。干旱处理2周大的幼苗后，转基因株系明显比野生型更抗旱，存活率高于野生型，并且失水率低于野生型。同时，转基因株系积累了较少的丙二醛（MDA），超氧化物歧化酶（SOD）活性和谷胱甘肽（GSH）含量也高于野生型。这些结果表明，柠条锦鸡儿CkLEA1基因在种子萌发阶段提高了拟南芥对盐和渗透胁迫的耐受性，并且提高了转基因拟南芥幼苗生长阶段对干旱胁迫的抵抗能力。

Heterologous of CkLEA1 Gene Enhanced Tolerance to Abiotic Stress in Arabidopsis

Plants are exposed to different abiotic stresses that affect growth, development, and productivity. Previous studies have shown that late embryogenesis abundant (LEA) proteins play protective roles in plant adaptation to abiotic stresses. Based on previous study, CkLEA1 (GenBank accession number: KC309408), a LEA gene isolated from Caragana korshinskii, was transferred into Arabidopsis. The transgene expression was detected by quantitative real-time PCR and three independent transgeniclines with different transgene expression level were selected for further analysis. CkLEA1 transgenic lines germinated more quickly than the wild-type on medium containing 200 mmol/L NaCl or 400 mmol/L mannitol. After drought stress treatment, the CkLEA1 transgenic lines were more resistance to drought stress and exhibited a significantly higher survival ratio. Meanwhile, transgenic lines lost water slow than the wild-type under dehydration treatment, accompanied with less accumulation of malondialdehyde (MDA) and increased superoxide dismutase (SOD) activities as well as glutathione (GSH) content. Taken together, these results indicated that CkLEA1 improved tolerance to salt and osmotic treatments during seed germination and increased tolerance to drought stress during seedling growth.