VC三菌种一步发酵方法的构建与研究

就维生素C微生物一步发酵方法进行了探索，构建了酮古龙酸杆菌、氧化葡萄糖酸杆菌和芽孢杆菌三菌混菌一步发酵的方法。研究发现，植物内生芽孢杆菌可以与酮古龙酸杆菌配合，促进酮古龙酸杆菌生长和产酸。在有山梨醇存在的条件下酮古龙酸杆菌及其伴生菌能够快速地生长增殖，植物内生芽孢杆菌在发酵的10h中不断消耗山梨醇。5L的发酵罐中，酮古龙酸杆菌、氧化葡萄糖酸杆菌和植物内生芽孢杆菌三菌混菌一步发酵在恒定的30℃温度，600r/min搅拌速度和1.5vvm通气条件下，补料发酵过程中醇酸质量转化率达到了81.89%，在分批发酵过程中，醇酸质量转化率达到了87.90%，进一步优化了维生素C生产工艺。

Analysis on and Construction of the VC One Step Fermentation of Three Strains

The aim is to analyze the vitamin C one-step fermentation process, and construct one step fermentation system including three strains of K. vulgare, G. oxydans and B. endophyticus. It was found that B. endophyticus could promote the growth and 2-KGA production of K. vulgare. They could grow fast under D-sorbitol, and 10 hours later of the fermentation process, B. endophyticus could consume D-sorbitol constantly. In the 5L fermenter, when the temperature was 30℃, stir speed was 600r/min, and the ventilation was 1.5vvm, the conversion rate of one step fementation of K. vulgare, G. oxydans and B. endophyticus was higher. In the fed-batch fermentation process, the mass conversion rate reached 81.89%, and in the batch fermentation process, the mass conversion rate reached 87.90%, it means the production process was optimized futher.