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Immunocytochemical study of the distribution of cytoskeletal proteins in neurons from mouse embryo spinal cord in culture
Authors:G. G. Skibo  O. L. Berezovskaya  L. M. Koval
Abstract:Monoclonal FITC and Rhodamine-labeled antibodies were used to investigate distribution of tubulin, actin, and neurofilament protein with a molecular weight of 160 kDa in neurons from mouse embryo spinal cord cultivated in a monolayer. It was found that migration of tubulin from the neuronal soma occurs during the course of nerve cell development. The extent to which neuronal processes became filled with tubulin varied according to the stage of cell differentiation. Neuronal actin concentration was negligible and was found at points along the processes close to focal contacts with lining cells. The main body of neurofilament protein is concentrated in the neuronal soma and proximal portions of neurites.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 20, No. 5, pp. 618–623, 1988.
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