Antigen Retrieval Using pH 3.5 Glycine-HCI Buffer or Urea Solution for Immunohistochemical Localization of Ki-67 |
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| Authors: | Shan-Rong Shi Benjaporn Chaiwun Lillian Young Ashraf Imam Richard J. Cote Clive R. Taylor |
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| Affiliation: | a Department of Pathology, University of Southern California School of Medicine, Los Angeles, California |
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| Abstract: | A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections. |
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