Purification of rabbit lacrimal gland plasma membranes by aqueous two-phase affinity partitioning |
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Authors: | Lars Ekblad Bengt Jergil J Peter Gierow |
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Abstract: | We describe the purification of lacrimal gland plasma membranes by affinity partitioning using a two-phase system containing polyethylene glycol and dextran in which wheat germ agglutinin conjugated to dextran is used as affinity ligand. When partitioning a microsomal fraction, the plasma membrane marker 5′-nucleotidase was obtained in the affinity ligand-containing bottom phase, whereas the endoplasmic reticulum marker NADH-ferricyanide reductase remained in the top phase. The affinity partitioning behaviour of components involved in exocytosis and cellular signalling was also examined. |
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Keywords: | Phosphatidylinositol 4-kinase |
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