| 斑点叉尾鲖源杀鲑气单胞菌无色亚种分离鉴定及药敏特性 |
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| 引用本文： | 杨移斌,胥宁,董靖,杨秋红,刘永涛,艾晓辉. 斑点叉尾鲖源杀鲑气单胞菌无色亚种分离鉴定及药敏特性[J]. 水生生物学报, 2017, 41(4): 787-792. DOI: 10.7541/2017.98 |
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| 作者姓名： | 杨移斌 胥宁 董靖 杨秋红 刘永涛 艾晓辉 |
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| 作者单位： | 中国水产科学研究院长江水产研究所,武汉 430223;淡水水产健康养殖湖北省协同创新中心,武汉 430223 |
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| 基金项目： | 公益性行业(农业)科研专项(201203085)资助(Supported by Special Fund for Agro-scientific Research in the Public Interest of China (201203085)) |
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| 摘 要： | 研究从患溃疡症斑点叉尾鲙中分离到一株致病性菌株ry01, 生理生化鉴定和16S rDNA基因序列分析表明ry01株为鲑气单胞菌无色亚种(Aeromonas salmonicida subsp. achromogenes)。人工感染该菌后发病鱼表现为与自然发病类似症状, 且从组织中再分离的细菌特性与原感染菌相同。腹腔注射后该菌株对斑点叉尾鲙的半数致死量为4.17×106 CFU/mL。菌株ry01对强力霉素及左氧氟沙星等高度敏感, 为斑点叉尾鲙该病防控提供了理论依据。
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| 关 键 词： | 斑点叉尾鲙 杀鲑气单胞菌无色亚种 分离鉴定 药敏特性 |
| 收稿时间： | 2016-06-22 |
ISOLATION AND IDENTIFICATION OF AEROMONAS SALMONICIDA SUBSP.ACHROMOGENES FROM ICTALUNES PUNCTATUS AND ITS ANTIMICROBIAL SUSCEPTIBILITY |
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| YANG Yi-Bin,XU Ning,DONG Jing,YANG Qiu-Hong,LIU Yong-Tao,AI Xiao-Hui. ISOLATION AND IDENTIFICATION OF AEROMONAS SALMONICIDA SUBSP.ACHROMOGENES FROM ICTALUNES PUNCTATUS AND ITS ANTIMICROBIAL SUSCEPTIBILITY[J]. Acta Hydrobiologica Sinica, 2017, 41(4): 787-792. DOI: 10.7541/2017.98 |
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| Authors: | YANG Yi-Bin XU Ning DONG Jing YANG Qiu-Hong LIU Yong-Tao AI Xiao-Hui |
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| Abstract: | The isolated bacterial strain ry01 fromIctalunes punctatus with ulcer disease was identified asAeromonas salmonicida subsp.achromogenes by analyzing biochemical reactions and 16S rDNA gene sequence. The ry01-injec-ted fish showed similar morphological and biochemical characteristics to those from naturally infected fish, supporting thatAeromonas salmonicida subsp.achromogenes was the pathogen toIctalunes punctatus. TheLD50was 4.17×106 CFU/mL according to the Reed-Muench method. The isolated bacterial strain ry01 was highly sensitive to doxycycline and levofloxacin. This study provides theoretical basis for the prevention and control the disease ofIctalunes punctatus. |
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| Keywords: | Ictalunes punctatus Aeromonas salmonicida subsp.achromogenes Isolation and identification Antimicrobial susceptibility |
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