Effect of ultrafine-grained titanium surfaces on adhesion of bacteria |
| |
Authors: | Vi Khanh Truong Stuart Rundell Rimma Lapovok Yuri Estrin James Y Wang Christopher C Berndt David G Barnes Christopher J Fluke Russell J Crawford Elena P Ivanova |
| |
Institution: | (1) Faculty of Life and Social Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria, 3122, Australia;(2) Department of Materials Engineering, Monash University, Clayton, Victoria, 3800, Australia;(3) ARC Centre of Excellence for Design in Light Metals, Clayton, Victoria, 3800, Australia;(4) IRIS, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria, 3122, Australia;(5) Centre for Astrophysics and Supercomputing, Swinburne University of Technology, P.O. Box 218, Hawthorn, Victoria, 3122, Australia |
| |
Abstract: | The influence of the ultrafine crystallinity of commercial purity grade 2 (as-received) titanium and titanium modified by
equal channel angular pressing (modified titanium) on bacterial attachment was studied. A topographic profile analysis of
the surface of the modified titanium revealed a complex morphology of the surface. Its prominent micro- and nano-scale features
were 100–200-nm-scale undulations with 10–15 μm spacing. The undulating surfaces were nano-smooth, with height variations
not exceeding 5–10 nm. These surface topography characteristics were distinctly different from those of the as-received samples,
where broad valleys (up to 40–60 μm) were detected, whose inner surfaces exhibited asperities approximately 100 nm in height
spaced at 1–2 μm. It was found that each of the three bacteria strains used in this study as adsorbates, viz. Staphylococcus aureus CIP 68.5, Pseudomonas aeruginosa ATCC 9025 and Escherichia coli K12, responded differently to the two types of titanium surfaces. Extreme grain refinement by ECAP resulted in substantially
increased numbers of cells attached to the surface compared to as-received titanium. This enhanced degree of attachment was
accompanied with an increased level of extracellular polymeric substances (EPS) production by the bacteria.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
| |
Keywords: | Titanium surfaces Equal channel angular pressing (ECAP) Bacterial adhesion Staphylococcus aureus Pseudomonas aeruginosa Escherichia coli |
本文献已被 SpringerLink 等数据库收录! |
|