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Comparison of some decontamination methods and growth media for isolation of mycobacteria from northern brook waters
Authors:E Livanainen  PJ Martikainen  M-L Katila
Institution:Laboratory of Environmental Microbiology, National Public Health Institute, Kuopio, Finland;Department of Clinical Microbiology, Kuopio University Hospital, Kuopio, Finland
Abstract:Two decontamination methods and five media were compared for the isolation of mycobacteria from brook waters of different physical, chemical and bacteriological characteristics. The decontaminants used were: 0.7 mol 1-1 NaOH followed by 50 g 1-1 oxalic acid and 0.9 mol 1-1 H2SO4 combined with 0.5 g 1-1 cycloheximide. The media compared were: Mycobacteria 7H11 agar with OADC enrichment (pH 6.6), glycerol egg (pH 6.5 and 5.5), and pyruvate egg (pH 6.5 and 5.5). All media contained cycloheximide, 0.5 g 1-1. The NaOH—oxalic acid method generally resulted in lower contamination and higher isolation of mycobacteria than the H2SO4-cycloheximide method. With the NaOH—oxalic acid method, all five media were equal in positivity rates but contamination was a problem on Mycobacteria 7H11 agar. Of the four egg media tested, the highest positivity rate (92% of the samples) was obtained on the pyruvate modification (pH 6.5), and the highest mean colony count of mycobacteria (900 cfu 1-1) on the glycerol modification (pH 6.5). Characteristics of water and sampling site had similar effects on the isolation frequencies of mycobacteria obtained by different combinations.
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