水母雪莲培养细胞对糖尿病小鼠血糖的影响

目的:探讨水母雪莲细胞培养物对糖尿病小鼠的降血糖作用。方法:将KKay糖尿病小鼠随机分为水母雪莲组、二甲双胍组和对照组,连续灌胃6周,每7 d称重并测量给药后3 h的空腹血糖,6周后测试药物的时-效关系及动物的糖耐量,最后检测甘油三酯、胆固醇、血清胰岛素等生化指标。结果:KKay小鼠饲喂雪莲7 d后,血糖值明显下降(P0.01),在此后的5周时间内,一直维持在较低血糖水平,其效应与阳性药物二甲双胍类似。连续给药6周后,雪莲组动物的糖代谢调节能力得到明显改善,甘油三酯、胆固醇、血清胰岛素较对照组显著降低。与二甲双胍组相比,雪莲的时-效关系表现为起效时间长、维持时间久等特点,即血糖值在给药后6 h明显下降,14 h达到最低,20 h左右逐渐恢复到给药前水平;而二甲双胍在给药后2 h即可见血糖明显下降,6 h血糖值最低,14 h后逐渐恢复到药前水平。结论:雪莲细胞培养物能有效降低KKay糖尿病小鼠的血糖水平,其作用机制可能与胰岛素的敏感性增加以及脂代谢调节有关。

Hypoglycemic Effects of the Cell Cultures of Sausurea medusa on KKay Diabetic Mice

Objective： To observe hypoglycemic activities of the cell cultures of Sausurea medusa on KKay dia betic mice. Methods： KKay mice were divided into S.medusa group, metformin group and control group, continuously administrated for 6 weeks. Body weight and fasting blood glucose were detected every seven days. The capa bility of glucose tolerance, time-effect relationship of S.medusa eel1, blood gluose, triglyceride, cholesterol, serum in sulin were determined at the 6th week. Results： From 7th day to 6 week, blood glucose in S.medusa group was deferent from control group（P〈0.01） and correspond to metformin group. After fed with the cultured cell of S.me dusa for 6 weeks, the capability of glucose tolerance was ameliorated. At the same time, triglyceride, cholesterol, serum insulin were significantly decreased. Compared with metformin, the time-effect relationship of S.medusa cell was characterized by a slow onset of drug and maintaining the role for a long time. In S.medusa cell group, blood glucose of mice began to decline after the administration 6 h, 14 h to achieve the minimum and 20 h or so grad- ually restored to the level before administration. Howere, in metformin group, the time was 2 h, 6 h and 14 h, re- spectively. Conclusion： The cell cuhures of S.medusa can decrease blood gluose on KKay mice and its mecha- nism may be correlated with increasing sensibility of serum insulin and/or regulating lipid metabolism.