MS2噬菌体衣壳蛋白与包装位点结合特异性及其生物学应用进展

MS2噬菌体为正义单链RNA噬菌体,基因组含有3569个核苷酸,编码成熟酶蛋白、衣壳蛋白、复制酶蛋白和裂解蛋白。MS2噬菌体复制酶编码基因5'端一个由19个碱基组成的茎环结构(又称包装位点)是衣壳蛋白二聚体与RNA相互作用的部位,二者相互作用形成的复合物是启动噬菌体自我包装的信号。MS2噬菌体衣壳蛋白与包装位点结合的特异性已被应用于RNA病毒核酸检测的标准物质、校准品和质控品的研究,实时动态监测活细胞内RNA的运动,以及RNA体内递送载体的研究等领域。

Biological Application of MS2 Bacteriophage Capsid Protein Binding Specificity of Packaging Site

MS2 is a single-stranded RNA bacteriophage of 3569 nucleotides that encodes four genes： the matu ration protein（A-protein）, the capsid protein, the lysisprotein, and the replicase protein. Package of the RNA ge nome by capsid protein is initiated by the binding of the capsid protein dimer to a specific 19-nucleotide stem loop region（pac site） located at the 5＇ terminus of the MS2 bacteriophage replicase gene. The specific binding of phage MS2 capsid protein and pac site have been applied for detecting of RNA in living cells as calibrator, RNA delivery vector and as standard, calibrator and quality control substance for RNA virus nucleic acid detection.