Paralog chromatography |
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Authors: | L M Kauvar P Y Cheung R H Gomer A A Fleischer |
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Institution: | Terrapin Technologies, Inc., San Francisco, CA 94103. |
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Abstract: | As a mixed mode ligand, a small peptide can mimic an antibody's paratope (antigen recognition site). This report describes the construction of a representative set of paratope analogs, or "paralogs," which can be conjugated to a chromatographic sorbent to combine desirable characteristics of traditional high-performance liquid chromatography columns with the specificity of a moderate affinity antibody. The broad utility of this novel set of protein separatory reagents is illustrated on the complex mixture of proteins in a yeast lysate. |
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