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Establishment of embryogenic suspension cultures of a wild relative of cotton (Gossypium klotzschianum Anderss.) |
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| Authors: | John J. Finer Ann A. Reilley Roberta H. Smith |
| Affiliation: | (1) Department of Agronomy, Ohio Agricultural Research and Development Center, The Ohio State University, 44691 Wooster, OH;(2) Department of Soil and Crop Sciences, Texas A&M University, 77843 College Station, Texas;(3) Present address: DNA Plant Technology Corporation, 182 Lewis Road, 95076 Watsonville, CA |
| Abstract: | Summary Maintainable, highly embryogenic suspension cultures of a wild relative of cotton (Gossypium klotzschianum Anderss.) have been obtained. Callus with no apparent organization was used to establish the liquid culture. Callus growth conditions as well as suspension medium composition were optimized. A visual selection scheme was beneficial for the maintenance of the embryogenic suspension. These liquid cultures have been maintained for over 10 mo. with no loss in embryogenic capacity. The somatic embryos developed after transfer of the embryogenic tissues to a hormone-free liquid medium. Salaries and research support were provided by State and Federal funds appropriated to OSU-OARDC. This is journal article No. 71-87. |
| Keywords: | Gossypium cotton embryogenesis suspension culture |
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