Metabolic significance and expression of Caenorhabditis elegans type II 3-oxoacyl-CoA thiolase |
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Authors: | Masanori Bun-ya Motohiro Maebuchi Summanuna H. Togo Takao Kurosawa Takashi Hashimoto Tatsuyuki Kamiryo |
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Affiliation: | (1) Faculty of Integrated Arts and Sciences, Hiroshima University, 739-8521 Higashi-Hiroshima Hiroshima, Japan;(2) Faculty of Pharmaceutical Sciences, Health Sciencess University of Hokkaido, 061-0293 Ishikari-Tobetsu, Japan;(3) Department of Pathology, Northwestern University Medical School, 60611-3008 Chicago, IL |
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Abstract: | The authors cloned the cDNA of the nematode Caenorhabditis elegans encoding a 44-kDa protein (P-44), which is similar to sterol carrier protein x (SCPx). Genomic DNA data and Northern blot analysis excluded the possibility of P-44 forming SCPx-like fusion protein. P-44 is required in the formation of bile acid in vitro from CoA esters of their enoyl-form intermediate in the presence of d-3-hydroxyacyl-CoA dehydratase/d-3-dehydrogenase bifunctional protein. Also, rat SCPx converts 24-hydroxy-form intermediate to bile acid under similar conditions. From this and other evidence, P-44 and SCPx were categorized as type II thiolase. The mRNA encoding P-44 was detected in every developmental stage of C. elegans: egg, larval stages, and adult. P-44, therefore, seems essential for the normal functioning of this organism. |
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Keywords: | Caenorhabditis elegans β -oxidation thiolase bile acid sterol carrier protein x (SCPx) peroxisome |
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