Differential Proteomics Analysis of Specific Carbonylated Proteins in the Temporal Cortex of Aged Rats: The Deterioration of Antioxidant System |
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Authors: | Qingsong Wang Xuyang Zhao Sizhi He Yashu Liu Mingrui An Jianguo Ji |
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Institution: | (1) The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871 Beijing, People’s Republic of China |
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Abstract: | Oxidative stress plays a pivotal role in normal brain aging and various neurodegenerative diseases, including Alzheimer’s
disease (AD). Irreversible protein carbonylation, a widely used marker for oxidative stress, rises during aging. The temporal
cortex is essential for learning and memory and particularly susceptible to oxidative stress during aging and in AD patients.
In this study, we used 2-DE, MALDI-TOF/TOF MS, and Western blotting to analyze the differentially carbonylated proteins in
the rat temporal cortex between 1-month-old and 24-month-old. We showed that the carbonyl levels of ten protein spots corresponding
to six gene products: SOD1, SOD2, peroxiredoxin 1, peptidylprolyl isomerase A, cofilin 1, and adenylate kinase 1, significantly
increased in the temporal cortex of aged rats. These proteins are associated with antioxidant defense, the cytoskeleton, and
energy metabolism. Several oxidized proteins identified in aged rat brain are known to be involved in neurodegenerative disorders
as well. Our findings indicate that these carbonylated proteins may be implicated in the decline of normal brain aging process
and provide insights into the mechanisms underlying age-associated dysfunction of temporal cortex. |
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