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Bioremediation of oil polluted aquatic systems and soils with novel preparation `Rhoder'
Authors:Valentina Murygina  Mikhail Arinbasarov  Sergey Kalyuzhnyi
Affiliation:(1) Department of Chemical Enzymology, Chemistry Faculty, Moscow State University, 119899 Moscow, Russia;(2) All-Russian Research Institute of Oil and Gas, 125422, Dmitrovskiy proyezd, 10, Moscow, Russia;(3) Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russia;(4) Department of Chemical Enzymology, Chemistry Faculty, Moscow State University, 119899 Moscow, Russia
Abstract:This paper summarises the experience accumulated duringthe field application of biopreparation `Rhoder' (solely or in a combinationwith preliminary mechanical collection of free oil) for remediation of oil polluted aquatic systems and soils in the Moscow region and Western Siberia during 1994–1999.It was demonstrated that `Rhoder' had a very high efficiency (>99%) for bioremediation of the open aquatic surfaces (100 m2 bay of the River Chernaya, two 5,000 m2 lakes in Vyngayakha) at initial level of oil pollution of 0.4–19.1 g/l. During remediation of the wetland (2,000 m2) in Urai (initial level of oil pollution of 10.5 g/l), a preliminary mechanical collection of oil was applied (75% removal) followed by a triple treatment with `Rhoder'. It resulted in an overall treatment efficiency of 94%. Relatively inferior results of bioremediation of the 10,000 m2 wetland in Vyngayakha (65% removal) and the 1,000 m2 marshy peat soil in Nizhnevartovsk (19% removal) can be attributed to the very high initial level of oil pollution (24.3 g/l and >750 g/g dry matter, respectively) aggravated by the fact that it was impossible to apply a preliminary mechanical collection of oil on these sites. A possible strategy for remediation of such heavily polluted sitesis discussed.
Keywords:bioremediation  hydrocarbon-degrading bacteria  indigenous microflora  oil  Rhodococcus
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