A peptide-mediated and hydroxyl radical HO*-involved oxidative degradation of cellulose by brown-rot fungi |
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| Authors: | Wang Wei Gao Peiji |
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| Institution: | (1) The State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100;(2) School of Chemical and Environmental Engineering, Jinan University, Jinan, 250002, The People's Republic of China |
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| Abstract: | A special low-molecular-weight peptide named Gt factor, was isolated and purified from the extracellular culture of brown-rot fungi Gloeophyllum trabeum via gel filtration chromatography and HPLC. It has been shown to reduce Fe3+ to Fe2+. Electron paramagnetic resonance (EPR) spectroscopy revealed Gt factor was able to drive H2O2 generation via a superoxide anion O2
.- intermediate and mediate the formation of hydroxyl radical HO. in the presence of O2. All the results indicated that Gt factor could oxidize the cellulose, disrupt the inter- and intrahydrogen bonds in cellulose chains by a HO. -involved mechanism. This resulted in depolymerization of the cellulose, which made it accessible for further enzymatic hydrolysis. |
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| Keywords: | atomic force microscopy brown-rot fungi cellulose degradation electron paramagnetic resonance hydrogen bonds hydroxyl radical HO |
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