| 酵母三杂交系统的应用研究进展 |
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| 引用本文： | 张浩,王全立,毛秉智. 酵母三杂交系统的应用研究进展[J]. 生物化学与生物物理进展, 1999, 26(6): 551-553 |
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| 作者姓名： | 张浩 王全立 毛秉智 |
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| 作者单位： | 军事医学科学院野战输血研究所，北京 100850;军事医学科学院野战输血研究所，北京 100850;军事医学科学院野战输血研究所，北京 100850 |
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| 摘 要： | 介绍了酵母三杂交系统的原理、应用、前景和存在的不足.在酵母双杂交基础上发展起来的酵母三杂交系统，将应用范围扩大到研究蛋白质-蛋白质、蛋白质-RNA、蛋白质-小分子药物间的相互作用.
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| 关 键 词： | 酵母三杂交系统 酵母双杂交系统，蛋白质间相互作用 |
| 收稿时间： | 1998-10-23 |
| 修稿时间： | : 1998-10- |
Advance in Application of Yeast Three-hybrid System |
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| ZHANG Hao,WANG Quan-Li and MAO Bing-Zhi. Advance in Application of Yeast Three-hybrid System[J]. Progress In Biochemistry and Biophysics, 1999, 26(6): 551-553 |
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| Authors: | ZHANG Hao WANG Quan-Li MAO Bing-Zhi |
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| Affiliation: | Beijing Institute of Transfusion Medicine, Beijing 100850, China;Beijing Institute of Transfusion Medicine, Beijing 100850, China;Beijing Institute of Transfusion Medicine, Beijing 100850, China |
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| Abstract: | Yeast three-hybrid system based on yeast two-hybrid system can be used in research of the interaction among three kinds of proteins and interaction among the proteins and RNA or interaction between protein and drug.Its theory,application and shortcoming were summarized. |
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| Keywords: | yeast three-hybrid system yeast two-hybrid system protein-protein interaction |
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