Protoplast culture and paclitaxel production by Taxus yunnanensis |
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Authors: | Luo Jian-Ping Mu Qing Gu Yue-Hua |
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Institution: | (1) School of Life Science, University of Science and Technology of China, Hefei, 230026, People's Republic of China; requests for offprints;(2) Department of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, People's Republic of China ( |
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Abstract: | Viable protoplasts of Taxus yunnanensis were isolated from friable, light yellow callus. Protoplast yield was dependent on callus age, with a maximum from 20-day-old
callus. Protoplasts were induced to undergo sustained divisions and to form cell colonies when cultured in medium consisting
of B5 salts, KM vitamin and organic components, 0.45 M fructose, 3.0 mg l-1 2,4-dichlorophenoxyacetic acid and 0.1 mg l-1 kinetin. The planting density was 2.5–3.0×105 protoplasts per ml of culture medium. Cell-free extract from callus enhanced protoplast division and the highest plating
efficiency was about 7%. Protoplast-derived colonies showed significant variations in both growth and paclitaxel content.
A negative correlation existed between paclitaxel accumulation in colonies and their growth to some extent (r = −0.4485).
Among 70 colonies isolated from the heterogeneous protoplast cultures, colony TY-7 accumulated the highest paclitaxel content.
Paclitaxel accumulation in colony TY-7 was not great enough to produce paclitaxel for commercial purposes, however, success
in inducing colony formation from T. yunnanensis protoplasts provides an opportunity to obtain cell lines with high paclitaxel productivity from mutagenized protoplast cultures.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Taxus yunnanensis protoplast-derived colonies paclitaxel |
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