Purification and characterization of protein Z from rabbit liver cytosol |
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| Authors: | S H Vincent B Holeman U Muller-Eberhard |
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| Affiliation: | 1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China;2. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, PR China;3. Science and Technology Literature Development Service Center, Jiangsu Information Institute of Science and Technology, Nanjing 210042, PR China |
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| Abstract: | Protein Z was purified from rabbit liver cytosol by affinity chromatography on oleic acid-agarose and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After removal of sodium dodecyl sulfate, the renatured protein was found to bind heme and bilirubin with a Kd of approximately 1 microM which produced large red shifts in their absorption spectra. On isoelectric focusing, rabbit protein Z exhibited two main bands with pI around 6.0. |
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