Analysis of 5-pyrrolidone-2-carboxylate ester by reverse phase high-performance liquid chromatography |
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| Authors: | E Bousquet V Guarcello M C Morale V Rizza |
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| Affiliation: | 1. Institute of Pharmaceutical Chemistry and Toxicology, University of Catania, Viale A. Doria 6, 95125 Catania, Italy;2. Department of Pharmacology, School of Medicine, University of Catania, Viale A. Doria 6, 95125 Catania, Italy;3. Division of Molecular Pharmacology, School of Medicine, University of Catania, Viale A. Doria 6, 95125 Catania, Italy;4. Department of Biochemistry, School of Medicine, University of Catania, Viale A. Doria 6, 95125 Catania, Italy |
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| Abstract: | A simple quantitative method for estimating nanomole concentrations of 5-pyrrolidone-2-carboxylic acid (PCA) in tissue homogenates from mouse has been developed using reverse-phase HPLC. PCA was detected as the 4-nitrophenacyl ester which has an absorption maximum at 263 nm, a relatively high stability, and excellent chromatographic separation and detectability. This method offers distinct advantages over other analytical procedures thus far employed for measuring PCA in that the 4-nitrophenacyl derivative of PCA can be readily prepared from deproteinized tissue homogenates and quantitated by HPLC within relatively short time intervals with good precision and specificity. |
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| Keywords: | To whom correspondence should be addressed. |
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