Development of EST-microsatellites from the cycad <Emphasis Type="Italic">Cycas rumphii</Emphasis>, and their use in the recently endangered <Emphasis Type="Italic">Cycas micronesica</Emphasis> |
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| Authors: | Angélica Cibrián-Jaramillo Thomas E Marler Rob DeSalle Eric D Brenner |
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| Institution: | (1) Genomics, The New York Botanical Garden, 200th St. and Kazimiroff Blvd, Bronx, NY 10458, USA;(2) Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park West at 79th St, New York, NY 10024, USA;(3) Western Pacific Tropical Research Center, College of Natural and Applied Sciences, University of Guam, UOG Station, Mangilao, Guam, 96923, USA |
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| Abstract: | We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged
from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform
conservation efforts and disentangle the biogeographic history of the genus. |
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| Keywords: | Cycad Cycas micronesica EST-STRs Invasives |
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