Sucrose requirements and lipid utilization during germination of interior spruce (Picea glauca engelmannii complex) somatic embryos |
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| Authors: | D. J. Carrier J. E. Cunningham D. C. Taylor D. I. Dunstan |
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| Affiliation: | (1) Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N OW9 Saskatoon, Saskatchewan, Canada;(2) Present address: Agricultural and Bioresource Engineering Department, University of Saskatchewan, 57 Campus Drive, S7N 5A9 Saskatoon, Saskatchewan, Canada |
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| Abstract: | Both somatic and excised zygotic embryos of interior spruce (Picea glauca engelmannii complex) required exogenous sucrose in the medium for germination in vitro. Over a period of 29 days on sucrose-containing medium germinants with roots and epicotyls developed from both kinds of embryo, and their content of linolenic acid (9,12,15-18:3) increased about six- to eightfold. Without added sucrose, embryos showed retarded growth or were necrotic, and the content of linolenic acid was barely detectable in their fatty acid profiles. Through14C-sucrose uptake studies, it was determined that germinants consumed only 25% of the sucrose available in a 1% (wt/vol) sucrose-containing medium. Since no radiolabelled fatty acids were detected, it appears that externally supplied sucrose was not used in the synthesis of lipids. Although sucrose was present during plantlet development, 72% of the initial lipids were consumed. To some extent, the plantlets appeared to be obligate storage lipid utilizers.Abbreviations 2,4-D 2,4-Dichlorophenoxyacetic acid - FAMEs Fatty acid methyl esters - HPLC High-performance liquid chromatography |
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| Keywords: | Carbohydrates Fatty acid Germination Somatic embryogenesis |
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