The structure of the oligosaccharides of alpha3beta1 integrin from human ureter epithelium (HCV29) cell line |
| |
Authors: | Lityńska Anna Pocheć Ewa Hoja-Lukowicz Dorota Kremser Elzbieta Laidler Piotr Amoresano Angela Monti Chiara |
| |
Institution: | Institute of Zoology, Jagiellonian University, Kraków, Poland. lita@zuk.iz.uj.edu.pl |
| |
Abstract: | There is a growing line of evidence that glycosylation of alpha and beta subunits is important for the function of integrins. Integrin alpha3beta1, from human ureter epithelium cell-line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin alpha3beta1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|