Development of a Simple PCR-based Marker for the Determination of Growth Habit in Vicia faba L. using a Candidate Gene Approach |
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Authors: | Carmen M Avila Salvador Nadal M Teresa Moreno Ana M Torres |
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Institution: | (1) Departamento de Genética, ETSIAM-UCO, Apdo. 3048, E-14080 Córdoba, Spain;(2) Area de Mejora y Biotecnología, CIFA-Alameda del Obispo, IFAPA-CICE (Junta de Andalucía), Apdo. 3092, E-14080 Córdoba, Spain |
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Abstract: | We have developed the first molecular marker suitable for the selection of determinate growth habit in faba bean using the
candidate gene approach. We obtained the sequences of TFL1/CEN like genes from public databases and designed primers on conserved domains. We used three cultivars with determinate growth
habit and four accessions (two cultivars and two lines) with indeterminate growth habit. All these genotypes are used in our
faba bean breeding program. A single monomorphic PCR fragment was obtained. A set of restriction enzymes was assayed. The
enzyme Hind1II produced a clear polymorphism between determinate and indeterminate genotypes. This new cleaved amplified polymorphism
(CAP) marker was tested using an F2 population contrasting for growth habit derived from the cross ‘Verde Bonita’ × 2N52. This marker showed 100% efficiency
in discriminating both types of genotypes. Moreover, the codominancy of this marker allows the detection of heterozygous individuals
facilitating the breeding process when pyramiding different genes. The perfect cosegregation of the marker with the trait
indicates that an orthologue of TFL1/CEN controls the growth habit in faba bean. This marker has been tested in all the genotypes used in our faba bean breeding program
as donors of the determinate growth habit. Therefore, it is expected to work well in all the crosses performed with these
parental lines as happens in the F2 tested. The CAPS marker developed in this work will be useful for Marker Assisted Selection programs. In addition, this marker
is useful for quality control to determinate the percentage of outsider seeds in commercial seed lots. Moreover, it is a valuable
tool to breeders when submitting new faba bean varieties for registration since the method allows guaranteeing that outsider
plants remains under the requested limit for registration. |
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Keywords: | CAPS Growth habit Marker Assisted Selection (MAS) Plant architecture Vicia faba |
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