Production of identical twin rabbits by micromanipulation of embryos

The research was conducted to improve micromanipulation procedures with rabbit embryos, including the production of genetically identical progeny. In the first experiment, embryos in different stages of development were used for micromanipulation by removing half of the blastomeres with a beveled aspirating pipette. Embryos 74-78 h postovulatory, in the late compacted morula or early blastocyst stage, were demonstrated to be best for micromanipulation. When embryos at this stage were halved, 77% (64/83) developed into blastocysts compared to 78% (65/83) for the intact control. In the second experiment, the survival of demi-embryos in original versus foreign zonae was tested. Young born from the demi-embryos transferred within original zonae (33%) were not significantly different (p greater than 0.05) from those transferred in foreign zonae (24%). Significantly more offspring, however, were obtained from intact control embryos (58%, p less than 0.01). In the third experiment, identical monozygotic twins were produced from Day 3 embryos, after modification of the aspirating pipette by further sharpening it to a fine point with a microforge. Thirty-four percent young (11) were obtained after microsurgery compared to 36% for intact control embryos transferred. Among the demi-embryos, a pair of albino and a pair of Dutch-belted young were identical twins.