Genetic diversity analysis and development of SCAR marker for detection of Indian populations of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">ciceris</Emphasis> causing chickpea wilt |
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Authors: | M Durai Sunil C Dubey Aradhika Tripathi |
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Institution: | (1) Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110 012, India; |
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Abstract: | Genetic diversity of the isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt collected from 12 states representing different agro-ecological regions of India was determined through
randomly amplified polymorphic DNA (RAPD) markers. The UPGMA cluster analysis grouped the isolates into eight categories showing
high magnitude of genetic diversity. Each group had the isolates from different states present in various agro-ecological
regions of India. Therefore, the groups generated through the RAPD analysis were not corresponding to area of the origin of
the isolates. The RAPD primers, namely, OPA 7 and OPA 11 produced Foc specific fragment of ≈1.3 kb and ≈1.4 kb, respectively in all the isolates. These fragments were eluted, purified, cloned
in pGEM-T Easy vector and sequenced. Primers were designed with sequence information of these two fragments using primer.3
software. Two sets of sequence characterized amplified region markers (SC-FOC 1 and SC-FOC 2) developed from the sequences
of these fragments were found to be specific to Foc and produced an amplicon of 1.3 and 1.4 kb, respectively. These set of markers were validated against the isolates of the
pathogen collected from different locations of India representing various races of the pathogen. They are non-specific to
the other Fusarium species, Rhizoctonia solani and R. bataticola. |
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