Production of lignocellulose-degrading enzymes employing <Emphasis Type="Italic">Fusarium solani</Emphasis> F-552 |
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Authors: | Stanislav Obruca Ivana Marova Petra Matouskova Andrea Haronikova Andrea Lichnova |
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Institution: | (1) Centre for Materials Research, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;(2) Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic |
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Abstract: | In this work, capability of Fusarium solani F-552 of producing lignocellulose-degrading enzymes in submerged fermentation was investigated. The enzyme cocktail includes
hydrolases (cellulases, xylanases, and proteinases) as well as ligninolytic enzymes: manganese-dependent peroxidase (MnP),
lignin peroxidase (LiP), and laccase (Lac). To our knowledge, this is the first report on production of MnP, LiP, and Lac
together by one F. solani strain. The enzyme productions were significantly influenced by application of either lignocellulosic material or chemical
inducers into the fermentation medium. Among them, corn bran significantly enhanced especially productions of cellulases and
xylanases (248 and 170 U/mL, respectively) as compared to control culture (11.7 and 29.2 U/mL, respectively). High MnP activity
(9.43 U/mL, control 0.45 U/mL) was observed when (+)-catechin was applied into the medium, the yield of LiP was maximal (33.06 U/mL,
control 2.69 U/mL) in gallic acid, and Lac was efficiently induced by, 2,2′-azino-bis-3-ethyltiazoline-6-sulfonate] (6.74 U/mL,
not detected in control). Finally, in order to maximize the ligninolytic enzymes yields, a novel strategy of introduction
of mild oxidative stress conditions caused by hydrogen peroxide into the fermentation broth was tested. Hydrogen peroxide
significantly increased activities of MnP, LiP, and Lac which may indicate that these enzymes could be partially involved
in stress response against H2O2. The concentration of H2O2 and the time of the stress application were optimized; hence, when 10 mmol/L H2O2 was applied at the second and sixth day of cultivation, the MnP, LiP, and Lac yields reached 21.67, 77.42, and 12.04 U/mL,
respectively. |
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