Gas7在大鼠海马和齿状回发育过程中的动态表达

目的研究生长休止蛋白7（Gas7）在大鼠海马和齿状回不同发育阶段的表达。方法采用免疫组织化学方法观察Gas7在SD大鼠胚胎第18d（E18）、新生（P0）、生后第7d（P7）、P14、P21和成年海马和齿状回中的表达和分布。结果在大鼠脑海马和齿状回部位的冠状切片上,Gas7免疫反应阳性产物主要表达在海马的锥体细胞、齿状回的颗粒细胞和门区的多形层细胞。随着发育的进程,在海马,Gas7较早表达在CA3区,其次是CA2和CA1区;在齿状回,Gas7在外臂的表达早于内臂,在颗粒细胞层的表达是按先外层后内层的顺序。在围生期,Gas7在海马和齿状回各区的表达逐渐增强,至P14达到高峰,后逐渐降低,至P21其表达强度和分布趋于恒定至成年水平。结论 Gas7在大鼠海马和齿状回发育过程中的动态表达具有时间和空间上的特异性,提示Gas7可能参与了海马和齿状回形态形成和功能成熟的调控。

Expression of Gas7 in rat hippocampal formation during development

Objective To study the expression of Gas7 during pre-and postnatal ontogenesis of rat hippocampus and dentate gyrus.Methods Immunohistochemistry was used to determine the expression of Gas7 in the rat HF from six developmental stages： embryonic day 18（E18）,postnatal day 0（P0）,P7,P14,P21 and adulthood.Results On the coronal sections of the embryonic and postnatal rat HF,Gas7 was expressed in the pyramidal cells of the hippocampus,granule cells of the dentate gyrus and the polymorphic layer cells of the hilus.During development,Gas7 positive pyramidal cells populated first the CA3 and then CA2 and CA1 regions respectively.In the dentate gyrus,the expression of Gas7 was detectable first in the supra-pyramidal blade and then the infra-pyramidal blade,and with outside-in expression gradient within the granule cell layer.The Gas7 expression was detected at low levels in E18 hippocampus and dentate gyrus,progressively increased to the highest level at P14,and then declined to the adult pattern by P21.Conclusion Gas7 expression in granule and pyramidal cells shows both position and time dependency,indicating that this protein is likely to be involved in morphogenesis and function maturation during hippocampus and dentate gyrus development.