| 褪黑素对小鼠MFC前胃癌细胞的survivin、caspase-3表达的影响 |
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| 引用本文： | 刘卉,宫喜,徐丽,蒋继浩,周瑞祥. 褪黑素对小鼠MFC前胃癌细胞的survivin、caspase-3表达的影响[J]. 中国组织化学与细胞化学杂志, 2011, 20(6): 549-553. DOI: 10.3870/zgzzhx.2011.06.002 |
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| 作者姓名： | 刘卉 宫喜 徐丽 蒋继浩 周瑞祥 |
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| 作者单位： | 福建医科大学人体解剖学与组织胚胎学系,神经生物学研究中心,福州,350004 |
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| 基金项目： | 国家自然科学基金资助(30971541);福建省科技厅重点项目(2009Y0023);福建医科大学校苗圃基金资助(2010MP024) |
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| 摘 要： | 目的探讨褪黑素（Melatonin,MLT）对小鼠前胃癌细胞（murine foregastric carcinoma,MFC）的生存素survivin和半胱天冬酶-3（cysteinyl aspartate-specific protease-3,caspase-3）表达的影响。方法使用不同浓度褪黑素处理培养的胃癌细胞,运用细胞免疫组化、实时荧光定量PCR、免疫印迹法、分光光度法等方法检测褪黑素在抑制小鼠MFC前胃癌细胞增殖过程中对细胞survivin、caspase-3的表达影响。结果与空白对照组相比,6mmol/L浓度褪黑素干预组的胃癌细胞survivin表达下调,caspase-3蛋白表达上调,并呈剂量依赖性。结论褪黑素能够降低胃癌细胞survivin的表达,进而激活caspase-3的活性,是褪黑素体外抑制肿瘤细胞增殖的作用机制之一。
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| 关 键 词： | 褪黑素 胃癌细胞 生存素 半胱天冬酶-3 |
Effect of melatonin on survivin and caspase-3 expression in murine foregastric cancer cells |
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| Liu Hui,Gong Xi,Xu Li,Jiang Jihao,Zhou Ruixiang. Effect of melatonin on survivin and caspase-3 expression in murine foregastric cancer cells[J]. Chinese Journal of Histochemistry and Cytochemistry, 2011, 20(6): 549-553. DOI: 10.3870/zgzzhx.2011.06.002 |
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| Authors: | Liu Hui Gong Xi Xu Li Jiang Jihao Zhou Ruixiang |
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| Affiliation: | (Department of Human Anatomy & Histology and Embryology,Neurobiology Research Center,Fujian Medical University,Fuzhou 350004,China) |
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| Abstract: | Objective To investigate the effect of melatonin on survivin and caspase-3 expression in murine foregastric cancer(MFC) cells.Methods MFC cells were treated with different concentrations of melatonin,and then we used immunocytochemistry,real-time fluorescence quantitative PCR,Western blot,spectrophotometry and other methods to detecte the effect of MLT on the cytokines of survivin and caspase-3 expression in MFC cells during their proliferation.Results Compared with the blank control,the 6mmol/L concentration of MLT down-regulated the expression of survivin and up-regulated caspase-3 expression in a dose dependent manner.Conclusion MLT can down-regulate the expression of survivin and activate the activity of caspase-3 protein in MFC cells,which is one of the anti-tumer mechanisms of melatonin in vitro. |
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| Keywords: | Melatonin Gastric cancer cell Survivin Caspase-3 |
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