几种念珠菌DNA总含量的流式细胞分析

应用流式细胞术(FCM)对处于稳定生长阶段的念珠菌属(Candida)的7种8株念珠菌进行了DNA总含量的流式细胞(FCM)分析。这8株念珠菌是:白念珠菌(C.albicans)2株,热带念珠菌(C.tropicalis),克柔念珠菌(C.krusei),近平滑念珠菌(C.parapsiolosis),乳酒念珠菌(C.kefyr),白念珠菌星形变种(C.stellatoidea),即血清B型白念珠菌,季也蒙念珠菌(C.guilliermondii)各一株。应用EB一步插入法染色,用鸡红细胞(CRBC)作为内参标准进行DNA总含量测定。分析结果表明:稳定生长阶段的组方图上,大多数念珠菌细胞处于DNA合成周期的G_0/G_1期;DNA总含量有明显的种间和种内差异。

FLOW CYTOMETRIC ANALYSIS OF TOTAL DNA PER CELL IN CANDIDA SPECIES

The total DNA per cell of eight Candida isolates in stationary-phase cultures were determined by flow cytometry, in which seven species were included, two strains of Candida albicans, one strain of C. tropicalis, C. krusei, C, parapsilosis, C. kefyr, C. stellatoidea (C. albicans serotype 5) and C. guilliermo ndii. One step staining of EB(ethidum bromide) was used to stain the cells and CRBC as internal parameter standard to assay total DNA per cell. Histograms of stationary-growth-phase Candida isolates demonstrate that the majority of popula. tion is in the G0/G1 phase of the DNA synthetic cycle. Inter-and Intra-specific differences in total DNA per cell were verified statistically by U test.