Isolation and characterization of three types of membranes from the cyanobacterium (blue-green alga) Synechocystis PCC 6714 |
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Authors: | Tatsno Omata Norio Murata |
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Institution: | (1) Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, 153 Tokyo, Japan |
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Abstract: | Three types of membranes were separated from the cyanobacterium Synechocystis PCC 6714 by mechanical disruption and density gradient centrifugation. Orange-colored membranes contained xanthophylls but little -carotene or chlorophyll a, green-colored membranes contained chlorophyll a, -carotene and xanthophylls, and another type of orange-colored membranes contained unknown xanthophylls. These membrane preparations were similar to those from Anacystis nidulans in pigmentation and buoyant density and were identified as purified preparations of the cytoplasmic membranes, thylakoid membranes and cell walls of Synechocystis PCC 6714, respectively.Abbreviations SDS
sodium dodecyl sulfate
- Tes
N-trishydroxymethyl]methyl-2-aminoethanesulfonic acid
- TLC
thin-layer chromatography |
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Keywords: | Synechocystis PCC 6714 Cell wall Cyanobacterium Cytoplasmic membrane Thylakoid membrane |
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