Stomatal characteristics during micropropagation of Wrightia tomentosa |
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Authors: | P Joshi N Joshi S D Purohit |
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Institution: | (1) Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, 313001, India |
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Abstract: | A deviation from usually found characteristics of stomata in Wrightia tomentosa was noted during in vitro propagation. Increase in stomatal frequency in leaves of plants grown in vitro was observed with 29.4 % malformed stomata. The stomata were spherical, wide open, did not close in detached leaves even
after 3 h. The leaves exhibited 93.4 % total water loss during 3-h period. Stomatal frequency, percentage of malformed stomata
and rate of water loss declined in subsequent rooting phase. Nevertheless, for high survival rate plantlets were hardened
under gradually decreasing air humidity either in partially opened glass bottles containing Soilrite™ moistened with 1/4 Murashige and Skoog nutrients or in pots covered with polyethylene bags. The stomatal characteristics
of hardened plants were comparable to seedlings. Survival rate was more than 95 %. |
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Keywords: | ex vitro transfer hardening in vitro culture stomatal frequency water relations |
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