An efficient and novel plant selectable marker based on organomercurial resistance |
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Authors: | Young Im Choi Eun Woon Noh Hyo Shin Lee Mu Seok Han Jae Soon Lee Kwan Sam Choi |
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Institution: | (1) Biotechnology Division, Korea Forest Research Institute, 441-350 Suwon, Korea;(2) Department of Applied Science, Chungnam National University, 305-764 Daejeon, Korea |
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Abstract: | A selectable marker gene facilitates the detection of genetically modified plant cells during transformation experiments.
So far, these marker genes are almost exclusively of two types, conferring either antibiotic resistance or herbicide tolerance.
However, more selectable markers must be developed as additional transgenic traits continue to be incorporated into transgenic
plants. Here, we used mercury resistance, conferred by the organomercurial lyase gene, as a selectable marker for transformation.
The merB gene fromStreptococcus aureus was modified for plant expression and transferred to a hybrid poplar(Populus alba xPopulus glandulosa), using the stem segment-agrobacteria co-cultivation method. The transformed cells were selected on a callus-inducing medium
containing as little as 1 μM methylmercury. Subsequent plant regeneration was done in the presence of methylmercury. Resistance
to Hg was stably maintained in mature plants after two years of growth in the nursery. We suggest that this gene could serve
as an excellent selectable marker for plant transformation. |
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Keywords: | merB mercury organomercurial lyase plant transformation selectable marker |
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