Partitioning of Proteins using a Hydrophobically Modified Ethylene Oxide/SDS Aqueous Two-phase System |
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Authors: | Gang Deng Shan-Jing Yao Dong-Qiang Lin |
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Institution: | (1) Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, PC 310027, P. R. China |
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Abstract: | Summary A novel aqueous two-phase system containing hydrophobically modified ethylene oxide (HM-EO) and sodium dodecyl sulphate (SDS)
was developed to enhance the selectivity of protein partitioning in two phases. Phase diagrams of HM-EO/H2O and HM-EO/SDS/H2O were measured, and the mechanism of interaction between HM-EO polymer and the anionic surfactant sodium dodecyl sulphate
(SDS) was also discussed. It was found that the improvement of selectivity of protein partitioning was related to the increase
of electrostatic potential difference between the two phases because of the charged network formed by mixed micelles of HM-EO
and SDS in the bottom phase. With bovine serum albumin (BSA) and lysozyme as model proteins, some factors, such as pH, SDS
concentration, conductivity and temperature of the system, were investigated for the influences of protein partition in HM-EO/SDS/H2O systems. The results showed that the addition of SDS not only changed the phase behaviour, but also played an important
role in protein partitioning. |
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Keywords: | Aqueous two-phase system hydrophobically modified ethylene oxide mixed micellar aggregates phase diagram protein partitioning |
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