Ethylene and polyamine interactions in morphogenesis of <Emphasis Type="Italic">Passiflora cincinnata</Emphasis>: effects of ethylene biosynthesis and action modulators,as well as ethylene scavengers |
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Authors: | Leonardo Lucas Carnevalli Dias Dimas Mendes Ribeiro Claudete Santa Catarina Raimundo Santos Barros Eny Iochevet Segal Floh Wagner Campos Otoni |
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Institution: | 1.Plant Cell Biology Laboratory, Department of Botany,IB-University of S?o Paulo,S?o Paulo,Brazil;2.Department of Plant Biology, Plant Growth and Development Laboratory,Federal University of Vi?osa,Vi?osa,Brazil;3.Plant Biology Department, Plant Tissue Culture Laboratory, BIOAGRO,Federal University of Vi?osa,Vi?osa,Brazil |
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Abstract: | Ethylene is a plant hormone that is of fundamental importance to in vitro morphogenesis, but in many species, it has not been
thoroughly studied. Its relationship with polyamines has been studied mainly because the two classes of hormones share a common
biosynthetic precursor, S-adenosylmethionine (SAM). In order to clarify whether competition between polyamines and ethylene
influences in vitro morphogenetic responses of Passiflora cincinnata Mast., a climacteric species, different compounds were used that act on ethylene biosynthesis and action, or as ethylene
scavengers. Treatment with the ethylene inhibitor, aminoethoxyvinylglycine (AVG) caused a greater regeneration frequency in
P. cincinnata, whereas treatment with the ethylene precursor, 1-aminocyclopropane-1-carboxylic-acid (ACC) lessened regeneration frequencies.
The data suggested that levels of polyamines and ethylene are not correlated with morphogenic responses in P. cincinnata. It was ascertained that neither the absolute ethylene and polyamine levels, nor competition between the compounds, correlated
to the obtained morphogenic responses. However, sensitivity to, and signaling by, ethylene appears to play an important role
in differentiation. This study reinforces previous reports regarding the requirement of critical concentrations and temporal
regulation of ethylene levels for morphogenic responses. Temporal regulation also appeared to be a key factor in competition
between the two biosynthetic pathways, without having any effects on morphogenesis. Further studies investigating the silencing
or overexpression of genes related to ethylene perception, under the influence of polyamines in cell differentiation are extremely
important for the complete understanding of this process. |
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