大鼠体外循环模型的建立及改进

目的对现有大鼠体外循环模型予以改进,降低模型复制难度,并使之更适合用于研究体外循环对肺功能的影响。方法成年雄性SD大鼠16只,体重300～350g,各8只分别用于建模和供血。经右颈静脉、左股静脉引流,右股动脉人工灌注建立体外循环,血气分析监测内环境变化。实验过程中,保留大鼠自主呼吸,不进行机械通气。结果成功建成8只大鼠体外循环模型并按计划顺利脱离人工循环。体外循环期间转流量为70～80mL/（kg·min）,血流动力学监测和血气分析结果基本正常。结论进一步简化了建立大鼠体外循环模型的操作,最显著的改进之处在于避免了机械通气对大鼠肺功能潜在的不良效应,从而更加适合用于研究体外循环对肺功能的影响。

Establishment and Improvement of a Rat Model of Cardiopulmonary Bypass

Objective To improve and simplify the techniques in establishment of cardiopulmonary bypass models in rats,and to better serve the research of adverse effects of cardiopulmonary bypass on respiratory function.Methods 8 adult male SD rats,weighing from 300 to 350 g,were scheduled to establish a cardiopulmonary bypass model by draining from the left femoral and right jugular veins,and perfusion into the right femoral artery with cannulation.The hemodynamics and blood-gas analysis parameters were monitored during the procedure of cardiopulmonary bypass.Self-respiration was reserved and no mechanical ventilation was administered to the rats throughout the experiment.Results Partial cardiopulmonary bypass was successfully established and weaned from in all of the 8 rats.During the procedure,the cardiopulmonary bypass blood flow was 70 to 80 mL/（kg·min）,the mean arterial blood pressure was 60 to 80 mmHg,and the blood gas analysis parameters were maintained at similar to normal reference values.Conclusions Due to avoiding the mechanical ventilation,the models established in the present study can prevent any potential adverse effects of mechanical ventilation on the pulmonary function.Therefore,this model more suitable for studies of the effects of cardiopulmonary bypass on pulmonary function.