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A modified column for sperm isoelectric focusing |
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| Authors: | Hamid Rahi Prakash N. Srivastava |
| Abstract: | A modified U-shaped column is described for efficient isoelectric focusing of spermatozoa, other cells, and protein. The washed spermatozoa of the rabbit showed a PI of 4.4. After sonication, heads and tails focused at same pH, indicating similar and equal charge. |
| Keywords: | isoelectric focusing apparatus sperm protein density gradient |