Substrate Effect on 2,3-Butylene Glycol Production by Rhizopus nigricans and Penicillium expansum

Rhizopus nigricans and Penicillium expansum produced 2,3-butylene glycol which accumulated in natural and artificial media with time. Mycelial mats of P. expansum decreased the quantity of a diacetyl substrate and converted part of this substrate into acetylmethylcarbinol (AMC) and 2,3-butylene glycol. Mycelial mats of P. expansum also decreased AMC substrate with the formation of 2,3-butylene glycol. 2,3-Butylene glycol decreased slightly during incubation with the fungal mat. The formation of AMC was suppressed significantly by cysteine and ascorbic acid.